Azelastine emedicine

“Western blotting”: electrophoretic transfer of proteins from sodium dodecyl sulfatepolyacrylamine gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Promega Protein Guide: Tips and Techniques (1993) Promega Corp. (1984) Visualization of antigenic proteins on Western blots. (1997) Antigen retrieval immunohistochemistry: past, present and future. (1996) Quantitative radioimmunohistochemical measurements of p185(erbB2) in frozen tissue sections. (1995) A quickscore method of immunohistochemical semiquantitation: validation for oestrogen receptors in breast carcinomas. (1995) CerbB2 protein expression in ovarian tumors. (1994) Localization of EGF receptors in frozen tissue sections by azelastine emedicine antibody and biotinylated EGFbased azelastine emedicine techniques. (1995) Statistical power: a measure of the quality of a study. (1994) Statistical aspects of prognostic factor azelastine emedicine studies in oncology. (1995) Sample size: how many patients are necessary? 52 Measurement of IGF1 Receptor Content in Tissues and Cell Lines by Radioimmunoassay (RIA) and ELISA Techniques Eberhard P. Beck, Laura Sciacca, Giuseppe Pandini, Wolfram Jaeger, and Vincenzo Pezzino 1. Introduction The IGF1 receptor (IGF1R) belongs to the tyrosine kinase growth factor receptor family. It is structurally similar to, but distinct from, the insulin receptor, with which it shares a 70% homology. As expected, it crossreacts with insulin and, vice versa, insulin receptor crossreacts azelastine emedicine with IGF1. Numerous studies suggest that IGF1R is very important for mitogenesis and is essential for phenotype transformation, at least in rodents (1). In particular, the IGF1R has been described in human breast cancer (24) and ovarian cancer (5) tissues and in cultured human breast cancer cell lines (6,7).

The possibility of measuring IGF1 receptors in human tissues and cultured cell lines would certainly be of interest in several neoplastic disorders both for clinical investigators in oncology and for basic azelastine emedicine science researchers. To quantitate IGF1R levels, specific 125Ilabeled IGF1 binding to the receptor is generally used (8). Although the binding assay is sensitive and easy, there are several drawbacks. The azelastine emedicine binding assay can only detect active and unoccupied receptors Buy hydrochlorothiazide 25 mg tab without prescription. Scatchard plots are sometimes linear, but often curvilinear (8,9), which makes the determination of the receptor number difficult. Most important is that significant amounts of IGF binding proteins could interfere with an accurate quantitation of the IGF1R, because they can also bind 125Ilabeled IGF1. In the past years, we have set up two different methods that are readily applicable to the measurement of IGF1 receptors in tissues and cell lines. The first is a sensitive and specific radioimmunoassay (RIA) (10), and the second is a recently developed ELISA method for the azelastine emedicine IGF1 receptor. The potential azelastine emedicine utility of such techniques has already been described in studies of breast cancer (11) and ovarian cancer (5). Culture medium: modified Eagle’s medium (MEM) containing 10% fetal calf serum (FCS), 2 azelastine emedicine mmol/L glutamine, 1X nonessential amino acids, and 40 pg/mL gentamycin. Serumfree medium: culture medium without FCS, containing 0. 1% bovine serum albumin (BSA) RIA grade, and 10 pg/mL transferrin. 39: Ovarian Cancer: Methods and Protocols Edited by: J. 4, containing 2 mg/mL bacitracin, 1 mM phenylmethylsulfonylfluoride (PMSF), and 1% Triton X100.